脂多糖预刺激对大脑皮质星形胶质细胞和小胶质细胞NO分泌水平的影响

doi:10.3969/j.issn.1004-616x.2011.03.004

›› 2011, Vol. 23 ›› Issue (3): 176-180.doi: 10.3969/j.issn.1004-616x.2011.03.004

脂多糖预刺激对大脑皮质星形胶质细胞和小胶质细胞NO分泌水平的影响

苏芸1,谢泽锋2 ,辛岗1,许燕璇1,李康生1，*

1. 汕头大学医学院微生物学与免疫学教研室，广东汕头 515041 ； 2. 汕头大学医院第一附属医院，广东汕头 515041

收稿日期:2011-03-21 修回日期:2011-04-01 出版日期:2011-05-30 发布日期:2011-05-30
通讯作者: 李康生

Lipopolysaccharide-preconditioning influences the NO secretion from cultured cerebral astrocytes and microglia in vitro

SU Yun1， XIE Ze-feng2， XIN gang1，XU Yan-xuan1， LI Kang-sheng1，*

1. Department of Microbiology and Immunology, Shantou University Medical College, Shantou 515041; 2. The First Affiliated Hospital, Shantou University Medical College, Shantou 515041, Guangdong, China

Received:2011-03-21 Revised:2011-04-01 Online:2011-05-30 Published:2011-05-30
Contact: LI Kang-sheng

摘要/Abstract

摘要： 探讨脂多糖 (lipopolysaccharide，LPS)预刺激对大脑皮质星形胶质细胞和小胶质细胞NO分泌水平的影响。方法：体外分离、纯化、培养星形胶质细胞和小胶质细胞。培养细胞分设LPS预刺激组(先用10 ng/ml LPS预刺激18 h后，改用1 μg/ml的 LPS再刺激)，LPS 10 ng/ml单次刺激组，LPS 1 μg/ml单刺激组与对照组(不用LPS刺激)。分别于LPS刺激后6、24、48 h收集细胞培养上清，用硝酸还原酶法检测NO水平。结果：星形胶质细胞和小胶质细胞，LPS预刺激组在24 h后， NO水平增加，明显高于相应时间点LPS 1 μg/ml单次刺激组 (P均<0.05)；其中，星形胶质细胞分泌NO的时间较长，可持续到48 h，与相应单次刺激组比较差异具有统计学意义 (P<0.05)。结论： LPS体外预刺激神经胶质细胞，可促使细胞分泌NO的水平升高。

关键词: 星形胶质细胞, 小胶质细胞, 脂多糖, 预刺激, 一氧化氮

Abstract: To study the influences of lipopolysaccharide(LPS) preconditioning on NO secretion in cultured cerebral astrocytes and microglia. METHODS： Mice cerebral astrocytes and microglia were isolated and purified in vitro. Thereafter， these glial cells were divided into 4 groups： LPS-primed group， 10 ng/ml LPS treated group， 1 μg/ml LPS treated group and non-treated group. The LPS-primed group was preconditioned with 10 ng/ml of LPS for 18 hours. After the preconditioning， the cells received the second treatment with 1 μg/ml of LPS for 6 hours， 24 hours and 48 hours. Then cultured supernatants of all 4 groups were collected. NO level in the supernatants was determined by Griess method. RESULTS： Both in cultured astrocytes and microglia，NO level of the LPS-primed group was increased at 24 hours with LPS re-treatment compared with the 1 μg/ml LPS-stimulated group (P<0.05). Moreover， at 48 hours with LPS re-treatment， a long-lasting and significant enhancing effect was found in astrocytes (P<0.05). CONCLUSION： LPS preconditioning could enhance NO secretion ability in cultured cerebral astrocytes and microglia.

Key words: astrocyte, microglia, lipopolysaccharide, preconditioning, nitric oxide

苏芸1,谢泽锋2 ,辛岗1,许燕璇1,李康生1，*. 脂多糖预刺激对大脑皮质星形胶质细胞和小胶质细胞NO分泌水平的影响[J]. , 2011, 23(3): 176-180.

SU Yun1， XIE Ze-feng2， XIN gang1，XU Yan-xuan1， LI Kang-sheng1，*. Lipopolysaccharide-preconditioning influences the NO secretion from cultured cerebral astrocytes and microglia in vitro[J]. , 2011, 23(3): 176-180.

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脂多糖预刺激对大脑皮质星形胶质细胞和小胶质细胞NO分泌水平的影响

Lipopolysaccharide-preconditioning influences the NO secretion from cultured cerebral astrocytes and microglia in vitro

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